Total RNA was isolated from a wild type C57BL/6 mouse treated for 7 days with 4-hydroxytamoxifen (4OHT; Sigma) using Trizol reagent (Gibco BRL). Double stranded cDNA was generated from 10 ug total RNA and used to generate biotinylated cRNA using the Enzo BioArray High Yield Transcription Labelling Kit (Affymetrix). The cRNA was fragmented using the GeneChip Sample Cleanup Module (Affymetrix) and 10 ug were hybridized to GeneChip Mouse Expression Set 430 A oligonucleotide arrays (Affymetrix). Hybridizations were performed according to the manufacturers instructions using an Affymetrix Gene Chip Instrument System. The arrays were scanned on an Affymetrix GeneChip scanner. Keywords = Mouse Keywords = Affymetrix Keywords = Skin Keywords = Hair Keywords = Epidermis Keywords = Keratinocyte Keywords = B-catenin Keywords = Stem Cell
