|
| Status |
Public on Feb 15, 2018 |
| Title |
WT Room Temperature Brown fat, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Wild type mouse brown fat
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Brown fat
genotype: wild type
gender: Male
cold exposure: Ambient room temperature
|
| Treatment protocol |
Wild type and Zbtb7b knockout mice were kept at ambient room temperature (22C) or following cold exposure at 4C for 4 hrs. Brown adipose tissue was harvested for total RNA isolation and microarray analysis.
|
| Growth protocol |
Wild type and Zbtb7b knockout mice were maintained on standard rodent chow
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the Affymetrix Plus WT kit protocol (GeneChip® WT Plus Reagent Kit Manual P/N 703174 Rev. 2) from 400 ng total RNA.
|
| |
|
| Hybridization protocol |
Following labeling procedure, 2.53 ug of cDNA were hybridized at 48C on Mouse Gene ST 2.1 arrays, and were washed and stained using the Affymetrix GeneTitan system (software version 4.3.0.1592)
|
| Scan protocol |
Arrays were scanned using the Affymetrix GeneTitan system (software version 4.3.0.1592)
|
| Description |
Gene expression data from wild type liver, pool 1
|
| Data processing |
Data was analyzed using the limma and oligo Bioconductor packages implemented in the R-statistical language version 3.3.0. RMA was used to fit log2 transformed expression values
|
| |
|
| Submission date |
Jul 07, 2017 |
| Last update date |
Feb 15, 2018 |
| Contact name |
Jiandie Lin |
| E-mail(s) |
[email protected]
|
| Phone |
734-615-3512
|
| Organization name |
University of Michigan
|
| Department |
Life Sciences Institute
|
| Street address |
210 Washtenaw Avenue
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL17400 |
| Series (1) |
| GSE100924 |
Wild type and Zbtb7b knockout mouse brown adipose tissue |
|
