|
| Status |
Public on Jul 11, 2017 |
| Title |
4T1CH9 MURINE TNBC MEK1 |
| Sample type |
RNA |
| |
|
| Source name |
4T1CH9 TNBC
|
| Organism |
Mus musculus |
| Characteristics |
sample type: Triple negative breast cancer
|
| Treatment protocol |
Vehicle versus Trametinib (1mg/kg) treatment in vivo for 7 days
|
| Growth protocol |
MFP injection in vivo 5e4, growth and establishment for 10 days followed by 7 days of treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction, PureLink RNA Mini Kit
|
| Label |
Biotin
|
| Label protocol |
BIOTIN, as per manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
cDNA was hybridized to Affymetrix Mouse Genome 430PM strip arrays for 16 hours at 45ºC.Washing and staining of microarrays was performed using the GeneAtlas Fluidics Station (Affymetrix, Santa Clara, CA)
|
| Scan protocol |
GeneChips were scanned in a GeneAtlas Imaging Station (Affymetrix, Santa Clara, CA)
|
| Description |
Affymetrix MG-430PM
Mouse tumor ex vivo, bulk RNA
|
| Data processing |
RMA normalization method, GeneGo, GSEA
|
| |
|
| Submission date |
Jul 10, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Sathana Dushyanthen |
| E-mail(s) |
[email protected]
|
| Organization name |
Peter MacCallum Cancer Centre
|
| Street address |
305 Grattan St
|
| City |
Melbourne |
| ZIP/Postal code |
3000 |
| Country |
Australia |
| |
|
| Platform ID |
GPL11180 |
| Series (1) |
| GSE101093 |
Murine triple negative breast cancer |
|
