|
| Status |
Public on Jul 27, 2017 |
| Title |
Adult 10% cohort mortality 2.5SY/2.5SY M4 |
| Sample type |
RNA |
| |
|
| Source name |
Adult male collected at 10% cohort mortality, raised on the 2.5SY diet as a larvae and maintained on the 2.5SY diet as an adult
|
| Organism |
Drosophila melanogaster |
| Characteristics |
Sex: male
age: 10% cohort mortality
larval diet: 2.5SY
adult diet: 2.5SY
tissue: whole body
|
| Treatment protocol |
Adults in each age-class were flash frozen in liquid nitrogen and transferred to the -80 to await RNA extraction.
|
| Growth protocol |
Flies were raised on one of three diets as larvae (0.25SY, 1SY, or 2.5SY) These three diets differed only in the amount of sugar and yeast they contained. The 1SY diet was identical to the standard laboratory diet (70g yeast, 100g sugar, 20g agar, 15mL nipagine, and 3mL propionic acid per liter of water), while the 0.25SY and 2.5SY diets contained 25% and 250% as much sugar and yeast as the 1SY diet, respectively. Newly eclosed adults were split across these same three diets in a fully factorial design and maintained on their adult diet until their death. Samples were collected at 1 day post eclosion and at 10% and 90% cohort mortality.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction was performed using the Machery Nagel Nucleospin II kit (Machery and Nagel) following the manufacturers instructions. We extracted RNA from whole bodies of five flies per replicate, with four replicates per combination of sex, larval diet, adult diet and age (4 replicates x 2 sexes x 3 larval diets x 3 adult diets x 3 ages = 216 arrays)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using the Affymetrix 3' IVT-Express Labeling Kit (#901229) according to the standard Affymetrix protocol from 100 ug total RNA per sample.
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| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized to the Affymetrix Drosophila 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix 3000G scanner.
|
| Description |
7032-07-227
|
| Data processing |
The data was processed using the robust multi-array average (RMA) algorithm to perform background adjustment, quantile normalization and summarization
|
| |
|
| Submission date |
Jul 26, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Christina May |
| E-mail(s) |
[email protected]
|
| Organization name |
Wageningen University
|
| Street address |
Droevendaalsesteeg 1
|
| City |
Wageningen |
| State/province |
Gelderland |
| ZIP/Postal code |
6708 PB |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE101882 |
Gene expression of young, middle-aged and old Drosophila melanogaster exposed to different levels of larval and adult diet |
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