|
| Status |
Public on Apr 10, 2008 |
| Title |
wt_PFC, biological replicate 5 |
| Sample type |
RNA |
| |
|
| Source name |
prefrontal cortex
|
| Organism |
Mus musculus |
| Characteristics |
C57BL/6, Gender: male, Age: 8 weeks,
|
| Treatment protocol |
none
|
| Growth protocol |
8 weeks old male littemate mice
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA were performed according to the manufacturer's instructions. As a last step of the extraction procedure, the RNA was purified with the RNeasy Mini-kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
According to Affymetrix Standard Protocols
|
| |
|
| Hybridization protocol |
According to Affymetrix Standard Protocols
|
| Scan protocol |
According to Affymetrix Standard Protocols
|
| Description |
Gene expression data from wt mouse prefrontal cortex
|
| Data processing |
The CEL files were analyzed with ArrayAssist (version 5.0, Stratagene Inc). The raw intensity data of all PFC samples or HPC samples were first GCRMA normalized and log2 transformed. T-test with FDR correction were then conducted for differential expression analysis.
|
| |
|
| Submission date |
Mar 11, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Joseph Gogos |
| Organization name |
Columbia University
|
| Department |
Physiology
|
| Street address |
630 W 168 st P&S 11-519
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE10784 |
Gene profile data from Df(16)A/+ and wild type littermates |
|
| Relations |
| Reanalyzed by |
GSE119085 |
