|
| Status |
Public on Aug 12, 2017 |
| Title |
grh mutant, rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Stage 16 embryos
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype: grhB37 mutant
developmental stage: Stage 16 embryos
|
| Growth protocol |
Embryos were aged to stage 16
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
RNA was extracted with Trizol and was incubated with Turbo DNAse. Four μg of total RNA was reverse transcribed with Superscript III reverse transcriptase (RT) using random primers.
|
| Label |
biotin
|
| Label protocol |
Samples were labeled at the Genomic Core Laboratory at Memorial Sloan-Kettering Cancer Center.
|
| |
|
| Hybridization protocol |
Samples were hybridized to the Affymetrix GeneChIP Drosophila Genome 2.0 Array at the Genomic Core Laboratory at Memorial Sloan-Kettering Cancer Center.
|
| Scan protocol |
Samples were scanned at the Genomic Core Laboratory at Memorial Sloan-Kettering Cancer Center.
|
| Data processing |
Data were normalized using the GCRMA frunction (R/bioconductor) with default settings. In addition MAS5 present/absent calls were computed (also using R/bioconductor)
|
| |
|
| Submission date |
Aug 11, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Christos Samakovlis |
| Organization name |
Stockholm University
|
| Department |
Department of Molecular Biosciences
|
| Lab |
Wenner-Gren Institute
|
| Street address |
Svante Arrhenius väg 20C
|
| City |
Stockholm |
| ZIP/Postal code |
S10691 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL1322 |
| Series (2) |
| GSE102549 |
Genome-wide identification of Grainy head targets in Drosophila [gene expression] |
| GSE102551 |
Genome-wide identification of Grainy head targets in Drosophila |
|
