|
| Status |
Public on Aug 24, 2017 |
| Title |
A549_Bj-1D-C |
| Sample type |
RNA |
| |
|
| Source name |
A549, 24h post H1N1 infection
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: A549
infection: H1N1
time: 24h
|
| Treatment protocol |
A549 cells were used to infect with influenza H1N1 virus (BJ501). A549 cells were washed and incubated with virus at a multiplicity of infection (MOI) of 1 for 1 h with FBS-free medium . After adsorption, the supernatant was aspirated and cells were cultured in medium supplemented with 2% FBS and 100 units penicillin-streptomycin ml-1 at 37°C in a humidified atmosphere of 5% (v/v) CO2 for indicate hours. 12 h with IAV WSN a nd SeV, o r 24 h with MDRV and HSV.
|
| Growth protocol |
A549 cells were purchased from the ATCC and cultured in DMEM/F-12(1:1) basic(Gibco) supplemented with 10% FBS and 100 units penicillin-streptomycin ml-1 at 37°C in a humidified atmosphere of 5% (v/v) CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
About 1ug total RNA were polyA tailed and labled with FlashTag™ Biotin HSR Labeling Kit. This kit can be used to label any RNA sample, including total RNA, severely degraded RNA, plant RNA, and low molecular weight RNA.
|
| |
|
| Hybridization protocol |
The labled RNA were hybridized for 16 hr at 48C on Affymetrix miRNA Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000.
|
| Description |
Gene expression data from A549 cells 24h post influenza H1N1(BJ501) infection.
|
| Data processing |
The data were analyzed with miRNA QCTool using Affymetrix default analysis settings and quantile as normalization method.
|
| |
|
| Submission date |
Aug 23, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Keyu Wang |
| E-mail(s) |
[email protected]
|
| Organization name |
State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology
|
| Street address |
Fengtai DONG DA Street NO.20
|
| City |
Beijing |
| ZIP/Postal code |
100071 |
| Country |
China |
| |
|
| Platform ID |
GPL21572 |
| Series (1) |
| GSE103009 |
Expression data from A549 cells infected with H1N1 virus |
|
