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Sample GSM2754456 Query DataSets for GSM2754456
Status Public on Oct 19, 2021
Title MB_FLI_GC5.5_skin_RNAseq
Sample type SRA
 
Source name skin
Organism Nothobranchius furzeri
Characteristics strain: GRZ
tissue: skin
age: 9 weeks
Sex: male
perturbation: DMSO
Extracted molecule total RNA
Extraction protocol Extraction was done as described in Baumgart et al. 2012. (PMID:22487494)
Library preparation was done using Illumina's TruSeq RNA Library Preparation Kit v2 following the manufacturer's instruction.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Data processing Sequence information was extracted in FastQ format using CASAVA software 1.8.2 data processing step.
Reads were mapped using STAR (2.4.1d).
Reads per gene were counted using featureCounts (1.4.6-p4).
Counts were normalized to RPKM values as described in Mortazavi et al. (2008).
Genome_build: genome v20150522 and annotation v20150922 can be downloaded from the Nothobranchius furzeri Information Network Genome Browser (http://nfingb.fli-leibniz.de/)
Supplementary_files_format_and_content: The Excel files include raw counts (sample_counts.xls) and RPKM values (sample_rpkms.xls) of all genes for each sample.
 
Submission date Aug 25, 2017
Last update date Oct 19, 2021
Contact name JenAge Project
E-mail(s) [email protected]
Organization name Leibniz Institute for Age Research - Fritz Lipmann Institute
Street address Beutenbergstr. 11
City Jena
ZIP/Postal code D-07745
Country Germany
 
Platform ID GPL17944
Series (1)
GSE103141 Sequencing of Nothobranchius furzeri (strain: GRZ) skin from Rotenone treated animals
Relations
BioSample SAMN07561744
SRA SRX3135939

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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