|
| Status |
Public on Mar 20, 2008 |
| Title |
U937_ROS_untreated_18h_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
differentiated U937 cells, ROS exposed, 18h
|
| Organism |
Homo sapiens |
| Characteristics |
Immortilised monocyte cell line
|
| Treatment protocol |
After differentiation, the U937 cells were starved overnight in phenol red free RPMI 1640 media with 0.5% FCS. The cells were then subjected to oxidative stress for 4 hours with the addition of H2O2 (100 uM). The media was then replaced with fresh complete growth media, and then incubated with/without curcumin (1uM) for a further 4hrs or 18 hrs, whereupon the cells were then harvested and total RNA extracted.
|
| Growth protocol |
The human monocytic cell line (U937) was obtained from the American Type Culture Collection (ATTC, Rockville, MD, USA) and maintained in complete growth media (RPMI 1640 media supplemented with 10% fetal calf serum (FCS), 2 mM L-glutamine, 100 U/ml penicillin and 100 g/ml streptomycin) at +37C in a humidified atmosphere with 5% CO2. U937 were differentiated into an adherent “macrophage-like” morphology by exposure to PMA (40 ng/ml) for 4 hours in complete growth media at +37C, then cultured for a further 48hrs.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy extraction of total RNA was performed according to the manufacturer's instructions (Promega).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol
|
| Scan protocol |
Standard Affymetrix protocol
|
| Description |
no additional information
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
|
| |
|
| Submission date |
Mar 19, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Paul Kirkham |
| E-mail(s) |
[email protected]
|
| Organization name |
Novartis Institutes for Biomedical Research
|
| Street address |
Wimblehurst Road
|
| City |
Horsham |
| State/province |
west Sussex |
| ZIP/Postal code |
RH12 5AB |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE10896 |
Impact of curcumin on human monocytes (U937 cells) exposed to oxidative stress |
|
| Relations |
| Reanalyzed by |
GSE119087 |
