|
| Status |
Public on Apr 02, 2009 |
| Title |
KT95-418-non-infected, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Tissue: aerial parts
|
| Organism |
Oryza sativa |
| Characteristics |
Age: 32th day after germination
|
| Treatment protocol |
Untreated
|
| Growth protocol |
RSV-innoculated or non-innoculated WuYun3 or KT95-418 rice plants were grown in greenhouse at 25±3C with a daily photoperiodic cycle of 16 hours light and 8 hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol 5 ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45 degree celsius on GeneChip Rice Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using GeneChip Scanner 3000
|
| Description |
Gene expression data from non-innoculated KT95-418
|
| Data processing |
GCOS1.0
|
| |
|
| Submission date |
Apr 02, 2008 |
| Last update date |
Apr 04, 2008 |
| Contact name |
Xiaoting Zhang |
| E-mail(s) |
[email protected]
|
| Phone |
86-591-83769714
|
| Organization name |
Fujian Agriculture and Forestry University
|
| Department |
Plant Protection Department
|
| Lab |
Plant Virology Institute
|
| Street address |
Jinshan
|
| City |
Fuzhou |
| State/province |
Fujian |
| ZIP/Postal code |
350002 |
| Country |
China |
| |
|
| Platform ID |
GPL2025 |
| Series (1) |
| GSE11025 |
Comparative transcriptional profiling of two contrasting rice genotypes in response to rice stripe virus infection |
|
