|
| Status |
Public on Sep 26, 2018 |
| Title |
d28 PorB 1 |
| Sample type |
RNA |
| |
|
| Source name |
spleen of a mouse given a third vaccination with PorB
|
| Organism |
Mus musculus |
| Characteristics |
tissue: spleen
timepoint: day 28
treatment: PorB
group: d28 PorB
|
| Treatment protocol |
Mice received vaccinations of 10ug ovalbumin, 10 ug PorB, or 10ug ovalbumin + 10ug PorB in 100uL PBS subcutaneously behind the neck on days -1, +13 and +27. Control mice received 100uL PBS only in a single vaccination. 24 hours post vaccination on the indicated date mice were euthanized and their spleens collected.
|
| Growth protocol |
6 week old C57BL/6 were obtained from Jackson Labs (Bar Harbor, ME).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Harvested spleens were homogenized, and RNA purified using and RNeasy kit (Qiagen, Hilden, Germany) according to the manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
Biotin labeling was performed using the WT Plus reagent kit (Affymetrix, Santa Clara, CA) according to the manufacturer's protocol.
|
| |
|
| Hybridization protocol |
The labeled, fragmented DNA was hybridized to the Affymetrix Mouse Gene 1.0 ST Array for 18 hours in a GeneChip Hybridization oven 640 at 45oC with rotation (60 rpm). The hybridized samples were washed and stained using an Affymetrix fluidics station 450.
|
| Scan protocol |
After staining, microarrays were immediately scanned using an Affymetrix GeneArray Scanner 3000 7G Plus.
|
| Description |
Gene expression data from the spleen of a mouse given a third vaccination with PorB.
|
| Data processing |
Raw Affymetrix CEL files were normalized to produce Entrez Gene-identifier-specific expression values using the implementation of the Robust Multiarray Average (RMA) in the affy Bioconductor R package and the Brainarray mogene10stv1mmentrezg R package (version 14.0.0).
|
| |
|
| Submission date |
Sep 27, 2017 |
| Last update date |
Jun 25, 2019 |
| Contact name |
Boston University Microarray and Sequencing Resource |
| E-mail(s) |
[email protected]
|
| Organization name |
Boston University
|
| Department |
Microarray and Sequencing Resource
|
| Street address |
72 East Concord Street, E631
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02118 |
| Country |
USA |
| |
|
| Platform ID |
GPL13730 |
| Series (1) |
| GSE104337 |
Modeling of the Response to a Multiple Vaccination Schedule Using the Adjuvant Neisseria meningitidis Porin B |
|
