RNA was isolated using magnetic beads from 25000 cels. Library preparation and amplification were performed as described by Gonzalez et al., 2010. Amplification was performed for 15 cycles.
Label
biotin
Label protocol
8 µg of cDNA was subsequently fragmented and labeled using GeneChip Mapping 250K Nsp Assay Kit (Affymetrix; catalog # 900766), according to manufacturer's instructions.
Hybridization protocol
cDNA was hybridized to the microarray for 16 hours at 45ºC, washing and staining of microarrays was performed using a GeneChip Fluidics Station 450 (Affymetrix, Santa Clara, CA)
Scan protocol
Arrays were scanned with GeneChip scanner GSC3000 (Affymetrix, Santa Clara, CA)
Description
WT drosophila S2 cells
Data processing
Arrays were processed in Bioconductor, using RMA background correction, quantile normalization and RMA summarization.
