|
| Status |
Public on Jun 21, 2018 |
| Title |
Obese-ALA rat 5 |
| Sample type |
RNA |
| |
|
| Source name |
rat tibialis anterior after 12 weeks of diet feeding
|
| Organism |
Rattus norvegicus |
| Characteristics |
Sex: male
genotype: Zucker obese
diet: ALA
tissue: tibialis anterior muscle
age: 18 weeks
|
| Treatment protocol |
Lean and obese control rats were given unrestricted access to the control diet (AIN-93G). The LA diet (AIN-93G + 10% safflower oil) and the ALA diet (AIN-93G + 10% flaxseed oil) were pair-fed in comparison to obese controls.
|
| Growth protocol |
Lean and obese Zucker rats (5 weeks) were received from Charles River. Rats were housed in environmentally controlled rooms with ad libitum access to water. Obese animals fed the ALA and LA supplemented diets were pair-fed to match for caloric intake of obese controls.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rats were anesthetized with an injection of pentobarbital sodium (60 mg/kg), and the red tibialis anterior muscle was excised and immediately placed in liquid nitrogen.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the standard GeneAtlas WT Plus Affymetrix protocol from 100 ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 5.5 ug of ss-cDNA was hybridized for 20 hr on Rat Transcriptome arrays incubated at 48°C. Strips were washed and stained using the Gene Atlas Fluidics Station.
|
| Scan protocol |
Rat Transcriptome arrays were scanned using the Gene Atlas Imaging Station
|
| Description |
Gene expression data from tibialis anterior following 12 weeks of diet
|
| Data processing |
Data were analyzed with the Bioconductor package 'oligo' (v1.32.0). Data were preprocessed (background correction and normalization) with the Robust Multichip Average algorithm ('rma' function in 'oligo') with default settings.
Signal intensity after RMA preprocessing
|
| |
|
| Submission date |
Oct 03, 2017 |
| Last update date |
Jun 22, 2018 |
| Contact name |
David M Mutch |
| E-mail(s) |
[email protected]
|
| Phone |
+1-519-824-4120
|
| Organization name |
University of Guelph
|
| Department |
Human Health & Nutritional Sciences
|
| Lab |
ANNU 308
|
| Street address |
50 Stone Road East
|
| City |
Guelph |
| State/province |
Ontario |
| ZIP/Postal code |
N1G 2W1 |
| Country |
Canada |
| |
|
| Platform ID |
GPL19271 |
| Series (1) |
| GSE104567 |
Expression data from tibialis anterior muscle of rats fed different fatty acid enriched diets |
|
