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Sample GSM281558 Query DataSets for GSM281558
Status Public on Apr 24, 2008
Title OZONE_1h
Sample type RNA
 
Channel 1
Source name Ozone_Control_Replication1
Organism Oryza sativa
Characteristics Healthy plants, untreated
Treatment protocol Healthy seedlings at 0 h, control
Growth protocol Plants were grown in growth chamber in soil for 14 days
Extracted molecule total RNA
Extraction protocol TRIZOL Reagent method
Label Cy5
Label protocol Standard Protocol of Agilent Low RNA Input Fluorescent Linear Amplification Kit
 
Channel 2
Source name Ozone_Treated_Replication1
Organism Oryza sativa
Characteristics Fumigated plants
Treatment protocol Fumigated for 1 h
Growth protocol Plants were grown in growth chamber in soil for 14 days
Extracted molecule total RNA
Extraction protocol TRIZOL Reagent method
Label Cy3
Label protocol Standard Protocol of Agilent Low RNA Input Fluorescent Linear Amplification Kit
 
 
Hybridization protocol Standard protocol of Gene Expression Hybridization Kit from Agilent Technologies.
Scan protocol Standard protocol of Gene Pix Scanner
Description Keyword = rice
Keyword = ozone
Data processing The GeneSpring Ver. 4 software was used for normalization.
 
Submission date Apr 13, 2008
Last update date Apr 23, 2008
Contact name Randeep Rakwal
E-mail(s) [email protected]
Fax +81 29 861 6066
Organization name AIST
Lab MST, HTRC lab
Street address AIST WEST, Onagawa 16-1
City Tsukuba
State/province Ibaraki
ZIP/Postal code 305-8569
Country Japan
 
Platform ID GPL892
Series (1)
GSE11157 Microarray analysis of rice plants fumigated with ozone

Data table header descriptions
ID_REF
VALUE Log10 ( Norm_CH2 / Norm_CH1)
Norm_CH1 Normalised Intensity of Raw_CH1
Norm_CH2 Normalised Intensity of Raw_CH2
RAW_CH1 Background subtracted Intensity of CH1
RAW_CH2 Background subtracted Intensity of CH2

Data table
ID_REF VALUE Norm_CH1 Norm_CH2 RAW_CH1 RAW_CH2
1 -1.032771172 399 37 380 35
2 -1.065118179 395 34 379 34
3 -0.903089987 24 3 23 2
4 -0.158362492 72 50 74 51
5 -0.017374096 51 49 52 50
6 -1.054357662 34 3 34 2
7 0.05299042 131 148 58 17
8 -0.141877159 61 44 61 42
9 -1.041392685 33 3 32 2
10 -0.392110465 37 15 37 13
11 -0.09300329 83 67 83 67
12 -0.078338773 103 86 106 86
13 -0.166331422 44 30 44 31
14 0.164586097 102 149 102 151
15 -0.109144469 63 49 64 51
16 -0.669006781 28 6 28 6
17 -0.110895659 71 55 72 52
18 -0.011429462 77 75 77 76
19 -0.421005313 29 11 29 11
20 0.026872146 47 50 49 49

Total number of rows: 22575

Table truncated, full table size 672 Kbytes.




Supplementary file Size Download File type/resource
GSM281558.gpr.gz 1.3 Mb (ftp)(http) GPR
Processed data included within Sample table

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