|
| Status |
Public on Oct 21, 2017 |
| Title |
HS adult 2 day, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Drosophila adult males aged 5-7 days after 2 days high-sugar feeding
|
| Organism |
Drosophila melanogaster |
| Characteristics |
strain: Canton-S
Sex: male
developemental stage: young adult (3-5 days)
fed with: high-sugar diet for 2 days
age at sacrifice: 5-7 days old
|
| Treatment protocol |
Flies were anesthetized with CO2, then homogenized on ice in the Tripure solution using a fresh RNAse-free pellet pestle for each biological replicate.
|
| Growth protocol |
See Musselman et al, 2011 (PMID 21719444) for diets. Adult Canton-S genotype flies were aged in single-sex vials until sacrifice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tripure extraction of total RNA was performed followed by RNeasy column to clean the total RNA.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol using 5 ug total RNA/biological replicate.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized on GeneChip Drosophila 2.0 arrays. GeneChips were washed and stained according to the recommended protocol.
|
| Scan protocol |
GeneChips were scanned using a scanner.
|
| Description |
hiad2d2
|
| Data processing |
dChip (2006 version) was used to extract probeset intensites and to normalize using the precise match/mismatch algorithm.
|
| |
|
| Submission date |
Oct 20, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Laura Palanker Musselman |
| E-mail(s) |
[email protected]
|
| Organization name |
Binghamton University
|
| Department |
Biological Sciences
|
| Lab |
S3 room 210
|
| Street address |
4400 Vestal Parkway East
|
| City |
Binghamton |
| State/province |
New York |
| ZIP/Postal code |
13902 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE105448 |
Expression data from adult wild-type male Drosophila melanogaster fed control or high-sugar diets |
|
