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Sample GSM2832298 Query DataSets for GSM2832298
Status Public on Feb 08, 2018
Title Sample 7_Arabidopsis csn5b-1 2day seeds
Sample type SRA
 
Source name Arabidopsis csn5b-1 2day seeds
Organism Arabidopsis thaliana
Characteristics ecotype background: Col-0
genotype/variation: csn5b-1
tissue: 2-day germinating seeds
Treatment protocol No treatment
Growth protocol Ripened seeds (8-weeks) of Col-0, csn5a-1, and csn1-10 that were collected on the same day from plants grown side-by-side at the same time were used. The 2-day germinating seeds, prepared with the same volume of dry seeds as above, were sterilized and incubate on cell culture wells with 0.5X liquid GM medium at 22oC under constant light for 2 days.
Extracted molecule polyA RNA
Extraction protocol RNAeasy Kit
stander Illumina mRNA-seq protocol
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Description Sample_2D5b-1_006
Data processing Bowtie for alignment, v1, default parameter
Cufflink for expression profiling, v2.1.0, default paremeter
Genome_build: TAIR10
Supplementary_files_format_and_content: "cuffdiff" output text file containing the normalized value and fold-changes
 
Submission date Oct 26, 2017
Last update date May 15, 2019
Contact name Bosheng Li
E-mail(s) [email protected]
Organization name SUSTC (current, may different with previous institution )
Department MCDB
Street address SUStech Huiyuan #1 406
City Shenzhen
State/province Guangdong
ZIP/Postal code 581055
Country China
 
Platform ID GPL13222
Series (1)
GSE106223 The COP9 Signalosome regulates seed germination by facilitating protein degradation of RGL2 and ABI5
Relations
BioSample SAMN07838716
SRA SRX3331756

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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