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Sample GSM28452

Query DataSets for GSM28452

Status

Public on Oct 08, 2004

Title

SCN2.2 Sample 6 (Exp#1-18 hr)

Sample type

RNA

Source name

Immortalized rat SCN (SCN2.2) cells

Organism

Rattus norvegicus

Extracted molecule

total RNA

Description

To determine whether immortalized cells derived from the rat SCN (SCN2.2) retain intrinsic rhythm-generating properties characteristic of the SCN, oscillatory properties of the SCN2.2 transcriptome were analyzed and compared to those found in the rat SCN in vivo using rat U34A Affymetrix GeneChips. SCN2.2 cells were expanded in 6-well plates. At 6-hour intervals cells from a single plate were harvested and pooled for total RNA extraction.
Keywords = Suprachiasmatic Nucleus / Circadian Pacemaker / Clock / Oscillator / SCN2.2 /.

Submission date

Aug 09, 2004

Last update date

May 27, 2005

Contact name

Gus John Menger

E-mail(s)

[email protected]

Phone

979-260-7309

Organization name

Texas A&M Health Science Center

Department

Anatomy and Neurobiology

Lab

Earnest

Street address

City

College Station

State/province

ZIP/Postal code

77843-1114

Country

USA

Platform ID

GPL85

Series (2)

GSE1654	Circadian Profiling of the Transcriptome in Immortalized Rat SCN Cells (3 biological replicates)
GSE4192	Circadian Profiling of the Transcriptome in Immortalized Rat SCN Cells

Data table header descriptions
ID_REF
VALUE	Signal intensity
ABS_CALL	Affymetrix detection flag
DETECTION P-VALUE	Detection p-values less then 0.05 are considered "Present."

Data table
ID_REF	VALUE	ABS_CALL	DETECTION P-VALUE
AFFX-MurIL2_at	38.3	A	0.724854
AFFX-MurIL10_at	53.2	A	0.382599
AFFX-MurIL4_at	25.8	A	0.51489
AFFX-MurFAS_at	55	A	0.195266
AFFX-BioB-5_at	330.5	P	0.021902
AFFX-BioB-M_at	538.2	P	0.000581
AFFX-BioB-3_at	207.6	M	0.046482
AFFX-BioC-5_at	922.6	P	0.000147
AFFX-BioC-3_at	579.4	P	0.000044
AFFX-BioDn-5_at	766.3	P	0.000662
AFFX-BioDn-3_at	3408.2	P	0.000169
AFFX-CreX-5_at	5969.1	P	0.000044
AFFX-CreX-3_at	8408.2	P	0.000044
AFFX-BioB-5_st	29.1	A	0.440646
AFFX-BioB-M_st	20.6	A	0.672921
AFFX-BioB-3_st	32.1	A	0.617401
AFFX-BioC-5_st	11.5	A	0.973889
AFFX-BioC-3_st	5.2	A	0.969024
AFFX-BioDn-5_st	39.4	A	0.39692
AFFX-BioDn-3_st	102.9	A	0.216524

Total number of rows: 8799

Table truncated, full table size 253 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM28452.EXP.gz	481 b	(ftp)(http)	EXP
GSM28452.RPT.gz	910 b	(ftp)(http)	RPT