|
| Status |
Public on Nov 11, 2017 |
| Title |
man skin biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
man skin
|
| Organism |
Homo sapiens |
| Characteristics |
subject status: healthy individual
gender: male
age: 22
tissue: skin biopsy
tissue subtype: man skin
molecule subtype: microRNA
|
| Treatment protocol |
Skin samples were stored in RNA-later at -20C before RNA isolation
|
| Growth protocol |
Skin biopsies were obtained from patients with androgenetic alopecia and healthy volunteers after obtaining informed consent.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
For RNA extraction skin tissues were washed in 0,01M PBS after RNA-later and homogenized mechanically with liquid nitrogen. Total RNA was isolated using RecoverAll™ Total Nucleic Acid Isolation kit (Cat# AM1975, Ambion, Vilnius, Lithuania)
|
| Label |
biotin
|
| Label protocol |
8 ul of total RNA samples containing from 130 to 270 ng microRNA were labeled with biotin using Affymetrix® Flash Tag™ Biotin HSR (Ref. 901913, Affymetrix, Santa Clara, CA, USA)
|
| |
|
| Hybridization protocol |
Hybridization on arrays in Affymetrix® miRNA 4.1 Array Strip (Affymetrix, Santa Clara, USA) using the GeneChip® GeneAtlas™ Hybridization and Stain Module (Ref. 902135) reagents (Affymetrix, Santa Clara, CA, USA) 48°C for 20 h
|
| Scan protocol |
Washing array strips on the GeneAtlas™ Microarray System Fluidic Station using the GeneChip® GeneAtlas™ Hybridization and Stain Module buffers and solutions (Ref. 902135) (Affymetrix, Santa Clara, CA, USA) and detecting on the Imaging Station of the GeneAtlas™ Microarray System
|
| Description |
Kr_man_skin
|
| Data processing |
Data were analyzed using Expression Console and Transcriptome Analysis Console 3.0 software (Affymetrix, USA).
affymetrix-algorithm-name = rma : dabg
affymetrix-algorithm-version = 1.0
affymetrix-array-type = miRNA-4_1
program-name = Expression Console
program-version = 1.4.0.38
|
| |
|
| Submission date |
Nov 10, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Anna Komina |
| E-mail(s) |
[email protected]
|
| Organization name |
Krasnoyarsk State Medical University
|
| Department |
Pathological physiology
|
| Street address |
Partizana Zheleznyaka
|
| City |
Krasnoyarsk |
| ZIP/Postal code |
660022 |
| Country |
Russia |
| |
|
| Platform ID |
GPL21572 |
| Series (1) |
| GSE106780 |
MicroRNA expression data obtained from skin samples of female pattern hair loss patients, healthy females and males |
|
