|
| Status |
Public on May 10, 2018 |
| Title |
mouse embryo Sox4_Sox11_ dko E14.5 brain, biological replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
mouse brain E14.5 (Brn4::Cre, conditional Sox4, conditonal Sox11)
|
| Organism |
Mus musculus |
| Characteristics |
strain: mixed 129SvJ/C57Bl/6J background
developmental stage: embryo E14.5
tissue: whole brain
genotype: Brn4::Cre, Sox4 flox/flox, Sox11 flox/flox
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA from whole mouse brains at E14.5 was performed according to the manufacturer's protocol. DNAseI was added to remove contaminating DNA and samples were cleaned up using the RNeasy Mini Kit according to Quiagen's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated aRNA was prepared in the amplification step using the GeneChip 3’ IVT Express Kit starting with 100ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, labeled aRNA was hybridized for 16h on a mouse genome 430 2.0 array. Hybridization, washing and staining of the arrays were done according to the standard Affymetrix GeneChip protocol (Version2).
|
| Scan protocol |
GeneChips were scanned using GC Scanner 3000 with G7 update.
|
| Description |
Gene expression data from E14.5 mouse brain (Sox4_Sox11_dko).
|
| Data processing |
The data were analyzed with Affymetrix Microarray Suite algorithm version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization procedure.
|
| |
|
| Submission date |
Nov 12, 2017 |
| Last update date |
May 10, 2018 |
| Contact name |
Michael Wegner |
| Organization name |
Friedrich-Alexander Universität Erlangen-Nürnberg
|
| Department |
Institut of Biochemistry
|
| Lab |
Michael Wegner
|
| Street address |
Fahrstr. 17
|
| City |
Erlangen |
| State/province |
Bavaria |
| ZIP/Postal code |
91054 |
| Country |
Germany |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE106786 |
Identification of SoxC-regulated genes during neurogenesis in the developing spinal cord [mouse] |
| GSE106788 |
Identification of SoxC-regulated genes during neurogenesis in the developing spinal cord |
|
