|
| Status |
Public on Sep 01, 2008 |
| Title |
Replicate Sall4 ChIP-chip in W4 ES cells |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Sall4 ChIP_2609102_532_pair
|
| Organism |
Mus musculus |
| Characteristics |
Germ-line competent mouse ES cell line W4
|
| Treatment protocol |
No treatment of cells prior to ChIP
|
| Growth protocol |
Cells were grown with Knockout DMEM supplemented with 15% fetal bovine serum, 1x non-essential amino acids, LIF, Glutamine, and beta-mercaptoethanol.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
ChIP-chip was performed starting with 10^8 cells and following the NimbleGen ChIP-chip protocol to extract IP DNA.
|
| Label |
Cy5
|
| Label protocol |
Standard Nimblegen ChIP labeling protocol
|
| |
|
| Channel 2 |
| Source name |
IgG ChIP_2609102_635_pair
|
| Organism |
Mus musculus |
| Characteristics |
Germ-line competent mouse ES cell line W4
|
| Treatment protocol |
No treatment of cells prior to ChIP
|
| Growth protocol |
Cells were grown with Knockout DMEM supplemented with 15% fetal bovine serum, 1x non-essential amino acids, LIF, Glutamine, and beta-mercaptoethanol.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
ChIP-chip was performed starting with 10^8 cells and following the NimbleGen ChIP-chip protocol to extract IP DNA.
|
| Label |
Cy3
|
| Label protocol |
Standard Nimblegen ChIP labeling protocol
|
| |
|
| |
| Hybridization protocol |
Standard Nimblegen hybridization kits and protocols were used
|
| Scan protocol |
Arrays were scanned by NimbleGen
|
| Description |
MM8 RefSeq Promoter array tiling array (2.5kb)
|
| Data processing |
Arrays were processed using Nimblegens standard protocols using NimbleScan for data extraction
|
| |
|
| Submission date |
Apr 30, 2008 |
| Last update date |
Jul 08, 2008 |
| Contact name |
Yupo Ma |
| E-mail(s) |
[email protected]
|
| URL |
http://www.nevadacancerinstitute.org
|
| Organization name |
Nevada Cancer Institute
|
| Lab |
4
|
| Street address |
1 Breakthrough Way
|
| City |
Las Vegas |
| State/province |
NV |
| ZIP/Postal code |
89135 |
| Country |
USA |
| |
|
| Platform ID |
GPL6797 |
| Series (1) |
| GSE11305 |
Sall4 ChIP-chip in mouse ES cell line W4 using NimbleGen MM8 RefSeq Promoter array (2.5kb) |
|
