|
| Status |
Public on Nov 29, 2017 |
| Title |
Male wild type mouse_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Male wild type mouse
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL6
tissue: whole liver
gender: Male
age: 10 to 11 weeks
genotype: wild type
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from liver tissues with Trizol reagents following manufacture's protocol. Extracted RNA quality was checked by the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).
|
| Label |
Cy3
|
| Label protocol |
Cy3 labeled cRNA was prepared from 0.1 ug RNA using the One-Color Low Input Quick Amp Labeling kit (Agilent) according to the manufacturer's instructions. cRNA was quantified with the NanoDrop ND-1000 Spectrophotometer.
|
| |
|
| Hybridization protocol |
The labelled cRNA was hybridized to the Agilent Whole Mouse Genome Microarray v2 8×60k following manufacture's protocol.
|
| Scan protocol |
Arrays were scanned using Agilent G2565A Microarray Scanner System.
|
| Description |
Gene expression in male wild type mouse liver
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 9.1.3.1 (Agilent Technology) using default parameters.
The raw signal intensities were log2 transformed and normalized by 75 percent tile shift using Gene spring GX software (Agilent Technology).
|
| |
|
| Submission date |
Nov 28, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Akihide Kamiya |
| E-mail(s) |
[email protected]
|
| Organization name |
Tokai University
|
| Street address |
143 Shimokasuya
|
| City |
Isehara |
| State/province |
Kanagawa |
| ZIP/Postal code |
259-1193 |
| Country |
Japan |
| |
|
| Platform ID |
GPL21163 |
| Series (1) |
| GSE107435 |
The effect of Bcl6 on liver sex difference |
|
