|
| Status |
Public on May 07, 2008 |
| Title |
48hr timepoint - Untreated control - biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
5 micrograms MCF7 total RNA
|
| Organism |
Homo sapiens |
| Characteristics |
untreated control
|
| Treatment protocol |
MCF7 cells were grown to 30-50% confluency and exposed to 10 nM E2 (or vehicle only) at 12, 24, and 48 hours. Each timepoint was performed in triplicate (ie, biological replicates).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was purified using the Qiagen RNAeasy kit according to the manufacturers instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 micrograms total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Standard Affymetrix procedure
|
| Scan protocol |
Standard Affymetrix procedure
|
| Description |
E2 treated or untreated (control) cells.
|
| Data processing |
Affymetrix Microarray Suite Version 5.0
|
| |
|
| Submission date |
May 06, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Lance David Miller |
| E-mail(s) |
[email protected]
|
| Phone |
65 6478 8100
|
| Fax |
65 6478 9060
|
| URL |
http://www.gis.a-star.edu.sg/internet/site/investigators.php?f=cv&user_id=7
|
| Organization name |
Genome Institute of Singapore
|
| Department |
Microarray and Expression Genomics
|
| Street address |
60 Bioplois Street, #02-01 Genome
|
| City |
Singapore |
| ZIP/Postal code |
138672 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE11352 |
Timecourse of estradiol (10nM) exposure in MCF7 breast cancer cells. |
|
| Relations |
| Reanalyzed by |
GSE119087 |
