|
| Status |
Public on Dec 31, 2008 |
| Title |
Pod vs Stem TCPSR01 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Pod_Replication 1
|
| Organism |
Arachis hypogaea |
| Characteristics |
Samples from pod tissues of FR458
|
| Treatment protocol |
No treatment
|
| Growth protocol |
Plants were field grown under pivot irrigation and tissue samples were harvested on 115 DAS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Qiagen Rneasy Plant Maxi Kit according to manufacturers protocol.
|
| Label |
Cy3
|
| Label protocol |
Standard Protocol of Agilent Low RNA Input Fluorescent Linear Amplification Kit
|
| |
|
| Channel 2 |
| Source name |
Stem_Replication 1
|
| Organism |
Arachis hypogaea |
| Characteristics |
Samples from stem tissues of FR458
|
| Treatment protocol |
No treatment
|
| Growth protocol |
Plants were field grown under pivot irrigation and tissue samples were harvested on 115 DAS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Qiagen Rneasy Plant Maxi Kit according to manufacturers protocol.
|
| Label |
Cy5
|
| Label protocol |
Standard Protocol of Agilent Low RNA Input Fluorescent Linear Amplification Kit
|
| |
|
| |
| Hybridization protocol |
Standard protocol of Gene Expression Hybridization Kit from Agilent Technologies.
|
| Scan protocol |
Standard protocol of GenePix 4000B Microarray Scanner
|
| Description |
Keyword = peanut
Keyword = tissue comparison
|
| Data processing |
Feature Extraction software Version 9.1 from Agilent Technologies
|
| |
|
| Submission date |
May 06, 2008 |
| Last update date |
May 08, 2008 |
| Contact name |
Kameswara Rao Kottapalli |
| E-mail(s) |
[email protected]
|
| Organization name |
USDA-ARS Lubbock & Texas Tech University
|
| Department |
Plant & Soil Science
|
| Lab |
Payton Lab
|
| Street address |
3810 4th Street
|
| City |
Lubbock |
| State/province |
TX |
| ZIP/Postal code |
79415 |
| Country |
USA |
| |
|
| Platform ID |
GPL6661 |
| Series (1) |
| GSE11365 |
Gene expression profiling in peanut using oligonucleotide microarrays |
|
