|
| Status |
Public on May 13, 2008 |
| Title |
wild type vs ace2 swi5 double gene deletion |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Wild Type
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
DY3419 wild type Saccharomyces cerevisiae
|
| Extracted molecule |
total RNA |
| Extraction protocol |
hot phenol extraction followed by pelleting thru CsCl
|
| Label |
Cy3
|
| Label protocol |
Agilent Two-Color LRILAK Labeling Protocol
|
| |
|
| Channel 2 |
| Source name |
ace2 swi5 double gene deletion
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
DY1143 ace2 swi5 double gene deletion Saccharomyces cerevisiae
|
| Extracted molecule |
total RNA |
| Extraction protocol |
hot phenol extraction followed by pelleting thru CsCl
|
| Label |
Cy5
|
| Label protocol |
Agilent Two-Color LRILAK Labeling Protocol
|
| |
|
| |
| Hybridization protocol |
Agilent Two-color Gene Expression Hyb/Wash protocol
|
| Scan protocol |
Microarray slides were scanned in an Agilent Technologies G2505B Microarray Scanner at 5 micron resolution. Images were quantified using Agilent Feature Extraction software version 8.5
|
| Description |
Microarray slides were scanned in an Agilent Technologies G2505B Microarray Scanner at 5 micron resolution. Images were quantified using Agilent Feature Extraction software version 8.5.
LOWESS normalized data obtained from log10 of processed Red signal/processed Green signal
|
| Data processing |
LOWESS normalized data obtained from log10 of processed Red signal/processed Green signal
|
| |
|
| Submission date |
May 07, 2008 |
| Last update date |
May 12, 2008 |
| Contact name |
David Stillman |
| E-mail(s) |
[email protected]
|
| Phone |
801 5815429
|
| Fax |
801 584517
|
| Organization name |
University of Utah
|
| Department |
Pathology
|
| Street address |
15 N. Medical Drive East, Room 2100
|
| City |
SALT LAKE CITY |
| State/province |
UT |
| ZIP/Postal code |
84112-5650 |
| Country |
USA |
| |
|
| Platform ID |
GPL2883 |
| Series (1) |
| GSE11380 |
Regulation by the homologous Ace2 and Swi5 factors of Saccharomyces cerevisiae |
|
