|
| Status |
Public on Jan 15, 2018 |
| Title |
33924902: FB stage V |
| Sample type |
RNA |
| |
|
| Source name |
Fruiting body stage V
|
| Organism |
Tuber melanosporum |
| Characteristics |
tissue: fruiting body
|
| Growth protocol |
Samples of T. melanosporum FBs were collected at different developmental stages in the field near the city of L’Aquila (Italy), using the methods described by Pacioni et al. in addition to the traditional use of trained dogs. All of the ascocarps were analysed morphologically and microscopically and classified by maturation stage according to the criteria defined by Zarivi et al. 2015. For structural analysis and maturation stage assessment, 8-10 µm cryostat sections of each FB were observed after Giemsa staining (1 min).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA were extracted from stages 3–6 (100 mg each) using TRIzol_ Reagent with the PureLink_RNA Mini Kit (Ambion by Life Technologies Carlsbad, CA, USA).
|
| Label |
Cy3
|
| Label protocol |
Labelling by NimbleGen Company
|
| |
|
| Hybridization protocol |
Hybridization by NimbleGen Company
|
| Scan protocol |
Scanning by NimbleGen Company
|
| Description |
This sampleis from stage V fruiting body (https://doi.org/10.1016/j.phytochem.2015.02.024)
|
| Data processing |
Expression values reflect across-array quantile normalization and gene calls generated using the Robust Multichip Average (RMA). For information on RMA, see Biostatistics 2003 Apr; 4(2): 249-264.
|
| |
|
| Submission date |
Dec 13, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Annegret Kohler |
| E-mail(s) |
[email protected]
|
| Phone |
+33 (0)383 394072
|
| Organization name |
INRAE
|
| Department |
UMR 1136
|
| Lab |
Interactions Arbres/Micro-organismes
|
| Street address |
Centre INRAE Grand Est Nancy
|
| City |
Champenoux |
| ZIP/Postal code |
54280 |
| Country |
France |
| |
|
| Platform ID |
GPL8982 |
| Series (1) |
| GSE108014 |
Gene expression in Tuber melanosporum fruiting bodies at different developmental stages |
|
