|
Status |
Public on May 30, 2008 |
Title |
Glucose-Glycerol early log phase 4 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
Wild type Listeria monocytogenes EGDe
|
Organism |
Listeria monocytogenes |
Characteristics |
Wild type Listeria monocytogenes EGDe grown in minimal medium with glucose to OD 600 nm 0.5 - early log phase and cells were harvested.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was isolated using RNeasy kit from Qiagen according to the manufacturers protocol with modifications as indicated in Marr et al., J Bacteriol. 2006 Jun;188(11):3887-901.
|
Label |
Cyanine 3
|
Label protocol |
Labeling performed exactly as described in Marr et al., J Bacteriol. 2006 Jun;188(11):3887-901
|
|
|
Channel 2 |
Source name |
Wild type Listeria monocytogenes EGDe
|
Organism |
Listeria monocytogenes |
Characteristics |
Wild type Listeria monocytogenes EGDe grown in minimal medium with glycerol to OD 600 nm 0.5 - early log phase and cells were harvested.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was isolated using RNeasy kit from Qiagen according to the manufacturers protocol with modifications as indicated in Marr et al., J Bacteriol. 2006 Jun;188(11):3887-901.
|
Label |
Cyanine 5
|
Label protocol |
Labeling performed exactly as described in Marr et al., J Bacteriol. 2006 Jun;188(11):3887-901
|
|
|
|
Hybridization protocol |
Hybridization performed exactly as described in Marr et al., J Bacteriol. 2006 Jun;188(11):3887-901
|
Scan protocol |
scanning software: ScanArray express software version: 3.0
|
Description |
Slides were scanned Using Perkin Elmer Scanarray.
|
Data processing |
VALUE: log2 transformed ratio of normalized medians normalization method: LOWESS algorithm
|
|
|
Submission date |
May 15, 2008 |
Last update date |
May 16, 2008 |
Contact name |
Biju Joseph Ampattu |
E-mail(s) |
[email protected]
|
Organization name |
University of Wuerzburg
|
Department |
Department of Microbiology
|
Street address |
Josef-Schneider Str 2
|
City |
Wuerzburg |
ZIP/Postal code |
97080 |
Country |
Germany |
|
|
Platform ID |
GPL6846 |
Series (1) |
GSE11459 |
Glycerol metabolism and PrfA activity in Listeria monocytogenes |
|
Data table header descriptions |
ID_REF |
|
Flags |
type of flag associated with the spot (absent/bad=0, good=1) |
Ch1 Median - B |
median channel 1 (median pixel intensity of the spot - background at 543nm) |
Ch2 Median - B |
median channel 2 (median pixel intensity of the spot - background at 633 nm) |
Ch1 N (Median-B) |
normalized channel 1 median |
Ch2 N (Median-B) |
normalized channel 2 median |
Ch2 N Ratio of Medians |
ratio of normalized channel 2 median to channel 1 median |
Unf_Value |
log2 transformed ratio of normalized channel 2 median to channel 1 median |
VALUE |
log2 transformed ratio of normalized channel 2 median to channel 1 median |