|
| Status |
Public on Nov 25, 2021 |
| Title |
Dif/Dl[J4] mutant larvae before injection |
| Sample type |
RNA |
| |
|
| Source name |
larvae before injection
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: whole body
genotype: Dif/Dl[J4]
age: 3rd inster larvae
|
| Treatment protocol |
3rd inster larvae before or 4hours after injection of Staphylococcus saprophyticus were homogenized in the Trizol solution (Invitrogen).
|
| Growth protocol |
Larvae for each genotype were reared in standard cornmeal medium at 25C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed using RNeasy kit (Qiagen).
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared using an IVT Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Labeled cRNAs were hybridized on Affymetrix Drosophila Genome 2.0 arrays.
|
| Scan protocol |
GeneChips were scanned using GeneChip Scanner 3000 (Affymeteix).
|
| Description |
control fly without bacteria
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings.
|
| |
|
| Submission date |
Dec 26, 2017 |
| Last update date |
Nov 25, 2021 |
| Contact name |
Naoyuki Fuse |
| Organization name |
Tohoku University
|
| Department |
Graduate School of Pharmaceutical Sciences
|
| Lab |
Laboratory for Molecular Genetics
|
| Street address |
Aoba 6-3, Aramaki, Aoba-ku
|
| City |
Sendai |
| State/province |
Miyagi |
| ZIP/Postal code |
980-8578 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE108510 |
Expression data of Gyc76C mutant larvae |
|
