|
| Status |
Public on Jan 24, 2018 |
| Title |
R4/imdca larvae, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Drosophila third instar larvae,with imd activation in the fat body, R4-GAL4/imdca
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: larvae
age: third instar
genotype: w;+/+;R4-GAL4,UAS-ImdCA/+
|
| Treatment protocol |
Third instar larvae were collected for each genotype and a total of 50 larvae for each replicate were used to proceed with Trizol RNA extraction.
|
| Growth protocol |
To induce innate immune responses, we generated a transgenic Drosophila line that allows cell-restricted activation of Imd and crossed it with a fat body specific driver (R4-GAL4). Larvae from R4-GAL4/imdca group and control genotypes (CA/+ and R4-GAL4/+) were raised on standard food at 25°C until they reached third instar stage and then collected for RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix GeneChip 3' IVT plus protocol from 100 ng total RNA (User Guide GeneChip® 3' IVT PLUS Reagent Kit, 2013-2015, Affymetrix).
|
| |
|
| Hybridization protocol |
12 ug of cRNA was used for fragmentation, fragmented cRNA was hybridized on GeneChip®Drosophila Genome 2.0 Array, washed and stained according to the Affymetrix protocol (User Guide GeneChip® 3' IVT PLUS Reagent Kit, 2013-2015, Affymetrix).
|
| Scan protocol |
Affymetrix GeneChip 3000 7G Scanner
|
| Description |
Gene expression data from R4-GAL4/imdca third instar larvae
|
| Data processing |
The data were analyzed with Expression Console (Affymetrix) using default analysis settings.
|
| |
|
| Submission date |
Jan 23, 2018 |
| Last update date |
Jan 24, 2018 |
| Contact name |
Saeideh Davoodi |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Alberta
|
| Department |
Medical Microbiology and Immunology
|
| Lab |
Dr. Edan Foley
|
| Street address |
116 st and 85 ave
|
| City |
Edmonton |
| State/province |
Alberta |
| ZIP/Postal code |
T6G2S2 |
| Country |
Canada |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE109470 |
Expression data from D.melanogaster larvae with a constitutively active Imd in the fat body |
|
