|
| Status |
Public on Nov 23, 2018 |
| Title |
HUVEC_siERG_day7 |
| Sample type |
RNA |
| |
|
| Source name |
HUVECs treated with siERG, day7
|
| Organism |
Homo sapiens |
| Characteristics |
cell: HUVEC
stimulation: siERG, day7
|
| Treatment protocol |
siRNAs were transfected with RNAiMAX Transfection Reagent (Thermo Fisher Scientific) following the manufacturer’s instructions.
|
| Growth protocol |
HUVECs were cultured in EGM-2 (Lonza) supplemented with 5% fetal bovine serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microgram of cRNA were hybridized for 16 hour at 45 degrees C. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChip was scanned using the Affymetrix GeneChip scanner 3000 7G.
|
| Description |
Gene expression data from HUVECs treated with siERG for 7 days
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Jan 25, 2018 |
| Last update date |
Nov 23, 2018 |
| Contact name |
Nao Nagai |
| Organization name |
Kumamoto University
|
| Street address |
2-2-1 Honjo, Chuo-ku
|
| City |
Kumamoto |
| ZIP/Postal code |
860-0811 |
| Country |
Japan |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE109662 |
Expression data in HUVECs treated with siERG, siFLI1, or both |
| GSE109696 |
ERG and FLI1 in endothelial cells |
|
