|
| Status |
Public on Nov 18, 2008 |
| Title |
LGMD2A patient_EXP-35_U133B |
| Sample type |
RNA |
| |
|
| Source name |
Proximal muscle (Deltoids)
|
| Organism |
Homo sapiens |
| Characteristics |
Gender: Male, Age: 48 years
Mutations: p.(Gln142X)/p.(Gln142X)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAPlus (Qbiogene) extraction of total RNA was performed according to the standard protocols for muscle tissue.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Affymetrix Protocol, May 5, 2005).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on HG-U133A/B Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Agilent GeneArray Scanner G2500A.
|
| Description |
LGMD2A patient_EXP-35_U133B
|
| Data processing |
Arrays were scanned and raw data were normalized using RMA (Robust Multichip Average)
|
| |
|
| Submission date |
Jun 04, 2008 |
| Last update date |
Nov 11, 2008 |
| Contact name |
Amets Saenz |
| E-mail(s) |
[email protected]
|
| Phone |
+34 943 00 70 61
|
| Fax |
+34 943 00 70 61
|
| Organization name |
Donostia Hospital
|
| Lab |
Unidad Experimental
|
| Street address |
Pº dr Begiristain s/n
|
| City |
San Sebastian |
| State/province |
Gipuzkoa |
| ZIP/Postal code |
20.014 |
| Country |
Spain |
| |
|
| Platform ID |
GPL97 |
| Series (1) |
| GSE11681 |
Expression profiling in LGMD2A muscles |
|
