the specimen contained > 80% tumor tissue and < 10% necrotic debris. 30-50 µg of frozen tissue sample were combined with 1 µl Trizol (Invitrogen) and dispersed using an Ultra-Turrax T25 tissue homogenizer (IKA). Total RNA was extracted according to the recommendations given by the Trizol protocol and further purified on RNeasy Mini spin columns (QIAGEN). Hybridization on an Affymetrix GeneChip HG-U133A array was carried out using standard Affymetrix protocols and equipment. Keywords = cancer Keywords = HNSCC Keywords = human Keywords = high density oligonucleotide array Keywords = comparative study Keywords = statistics & numerical data Keywords = sensitivity and specificity
Cross-platform reproducibility of oligonucleotide microarray expression profiles
Data table header descriptions
ID_REF
VALUE
normalized expression measurement value. Raw signal intensities were normalized applying the variance stabilization method (W. Huber et al., Bioinformatics 18 Suppl. 1, 2002).
VSN_LOG2
log2-transformed normalized expression measurement value
MAS5_VALUE
normalized expression measurement value obtained using the MAS5 algorithm
