|
| Status |
Public on Jun 27, 2008 |
| Title |
pkl Mutant Germinating Seeds With Uniconazole Treatment Set 1 of 6 |
| Sample type |
RNA |
| |
|
| Source name |
pkl Mutant Germinating Seeds With Uniconazole Treatment Set 1 of 6
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
Relative expression data from germinating seeds of pkl mutant treated with uniconazole (Upkl) after testa rupture and during endosperm rupture (radicle emergence).
|
| Biomaterial provider |
Joe Ogas
|
| Treatment protocol |
Treated with 10 nM uniconazole and 0.01% methanol (solvent) present in Murashige and Skoog Basal Medium with Gamborg's Vitamins solidified with agar.
|
| Growth protocol |
Seeds were germinated in a CU36L5 incubator under 24h illumination.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Hot Phenol with LiCl precipitation followed by RNeasy clean up (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Affymetrix protocol
|
| |
|
| Hybridization protocol |
Affymetrix protocol
|
| Scan protocol |
Affymetrix protocol
|
| Description |
Relative expression data from germinating seeds of Columbia (wt), the pkl mutant (pkl), Columbia plus uniconazole-P (Uwt) and the pkl-mutant plus uniconazole-P (Upkl). Each experimental condition (wt, pkl, Uwt and Upkl) has six biological replicates for a total of 24 chips.
|
| Data processing |
MAS 5.0
|
| |
|
| Submission date |
Jun 20, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Joe Ogas |
| E-mail(s) |
[email protected]
|
| Phone |
(765) 496-3969
|
| Fax |
(765)-494-7897
|
| URL |
http://www.purdue.edu/biochem/faculty/ogas.html
|
| Organization name |
Purdue University
|
| Department |
Biochemistry
|
| Street address |
175 S. University St.
|
| City |
West Lafayette |
| State/province |
IN |
| ZIP/Postal code |
47907-2063 |
| Country |
USA |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE11852 |
Effect of uniconazole on wt and pkl mutant germinating seeds |
|
| Relations |
| Reanalyzed by |
GSE118579 |
| Reanalyzed by |
GSE119083 |
