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Sample GSM300678 Query DataSets for GSM300678
Status Public on Dec 31, 2008
Title Control_liver_3
Sample type RNA
 
Source name Liver
Organism Mus musculus
Characteristics Strain: mixed
Tissue: liver
Age: 28 days
Growth protocol Standard housing, feeding, and water. Fasted for 5 hours prior to euthanasia.
Extracted molecule total RNA
Extraction protocol mirVana™ miRNA Isolation Kit (Ambion)
Label Biotin
Label protocol 100ng of total RNA per sample was amplified and biotinylated using the MessageAmp Premier kit (Ambion)
 
Hybridization protocol The hybridization was performed according to the Affymatrix manual using a Tecan HS4800 hybridization station (Tecan, Austria)
Scan protocol Slides were scanned by a ScanArray 4000 XL scanner (Packard Biochip Technologies, USA)
Description Hepatic gene expression data
Data processing Image analysis was carried out using the ImaGene 6.1.0 software (BioDiscovery, Inc., USA)
 
Submission date Jun 26, 2008
Last update date Aug 28, 2018
Contact name Joshua Friedman
E-mail(s) [email protected]
Organization name University of Pennsylvania School of Medicine
Department Pediatrics
Street address ARC 1007B, 3615 Civic Center Blvd.
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
 
Platform ID GPL1261
Series (1)
GSE11899 Gene expression in Dicer-deficient mouse liver
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE The quantified signals were normalized using the GC-RMA algorithm, with exclusion of probe sets absent in > samples.

Data table
ID_REF VALUE
AFFX-BioB-5_at 8.091870308
AFFX-BioB-M_at 9.589369774
AFFX-BioB-3_at 8.645210266
AFFX-BioC-5_at 9.524550438
AFFX-BioC-3_at 10.37650013
AFFX-BioDn-5_at 11.98349953
AFFX-BioDn-3_at 11.95549965
AFFX-CreX-5_at 14.71940041
AFFX-CreX-3_at 14.73950005
AFFX-r2-Ec-bioB-5_at 9.182869911
AFFX-r2-Ec-bioB-M_at 10.0703001
AFFX-r2-Ec-bioB-3_at 9.261980057
AFFX-r2-Ec-bioC-5_at 10.76780033
AFFX-r2-Ec-bioC-3_at 10.65939999
AFFX-r2-Ec-bioD-5_at 13.35449982
AFFX-r2-Ec-bioD-3_at 14.4673996
AFFX-r2-P1-cre-5_at 15.20549965
AFFX-r2-P1-cre-3_at 15.69919968
AFFX-r2-Bs-lys-3_at 3.064340115
AFFX-r2-Bs-thr-5_s_at 2.203860044

Total number of rows: 25995

Table truncated, full table size 594 Kbytes.




Supplementary file Size Download File type/resource
GSM300678.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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