|
Status |
Public on Dec 31, 2008 |
Title |
Control_liver_4 |
Sample type |
RNA |
|
|
Source name |
Liver
|
Organism |
Mus musculus |
Characteristics |
Strain: mixed Tissue: liver Age: 28 days
|
Growth protocol |
Standard housing, feeding, and water. Fasted for 5 hours prior to euthanasia.
|
Extracted molecule |
total RNA |
Extraction protocol |
mirVana™ miRNA Isolation Kit (Ambion)
|
Label |
Biotin
|
Label protocol |
100ng of total RNA per sample was amplified and biotinylated using the MessageAmp Premier kit (Ambion)
|
|
|
Hybridization protocol |
The hybridization was performed according to the Affymatrix manual using a Tecan HS4800 hybridization station (Tecan, Austria)
|
Scan protocol |
Slides were scanned by a ScanArray 4000 XL scanner (Packard Biochip Technologies, USA)
|
Description |
Hepatic gene expression data
|
Data processing |
Image analysis was carried out using the ImaGene 6.1.0 software (BioDiscovery, Inc., USA)
|
|
|
Submission date |
Jun 26, 2008 |
Last update date |
Aug 28, 2018 |
Contact name |
Joshua Friedman |
E-mail(s) |
[email protected]
|
Organization name |
University of Pennsylvania School of Medicine
|
Department |
Pediatrics
|
Street address |
ARC 1007B, 3615 Civic Center Blvd.
|
City |
Philadelphia |
State/province |
PA |
ZIP/Postal code |
19104 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE11899 |
Gene expression in Dicer-deficient mouse liver |
|
Relations |
Reanalyzed by |
GSE119085 |