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Sample GSM300681 Query DataSets for GSM300681
Status Public on Dec 31, 2008
Title Mutant_liver_1
Sample type RNA
 
Source name Liver
Organism Mus musculus
Characteristics Strain: mixed
Tissue: liver
Age: 28 days
Growth protocol Standard housing, feeding, and water. Fasted for 5 hours prior to euthanasia.
Extracted molecule total RNA
Extraction protocol mirVana™ miRNA Isolation Kit (Ambion)
Label Biotin
Label protocol 100ng of total RNA per sample was amplified and biotinylated using the MessageAmp Premier kit (Ambion)
 
Hybridization protocol The hybridization was performed according to the Affymatrix manual using a Tecan HS4800 hybridization station (Tecan, Austria)
Scan protocol Slides were scanned by a ScanArray 4000 XL scanner (Packard Biochip Technologies, USA)
Description Hepatic gene expression data
Data processing Image analysis was carried out using the ImaGene 6.1.0 software (BioDiscovery, Inc., USA)
 
Submission date Jun 26, 2008
Last update date Aug 28, 2018
Contact name Joshua Friedman
E-mail(s) [email protected]
Organization name University of Pennsylvania School of Medicine
Department Pediatrics
Street address ARC 1007B, 3615 Civic Center Blvd.
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
 
Platform ID GPL1261
Series (1)
GSE11899 Gene expression in Dicer-deficient mouse liver
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE The quantified signals were normalized using the GC-RMA algorithm, with exclusion of probe sets absent in > samples.

Data table
ID_REF VALUE
AFFX-BioB-5_at 8.678319931
AFFX-BioB-M_at 10.36779976
AFFX-BioB-3_at 9.389860153
AFFX-BioC-5_at 10.13020039
AFFX-BioC-3_at 11.06669998
AFFX-BioDn-5_at 12.76900005
AFFX-BioDn-3_at 12.66069984
AFFX-CreX-5_at 15.28339958
AFFX-CreX-3_at 15.28579998
AFFX-r2-Ec-bioB-5_at 9.831219673
AFFX-r2-Ec-bioB-M_at 10.75650024
AFFX-r2-Ec-bioB-3_at 9.825510025
AFFX-r2-Ec-bioC-5_at 11.39780045
AFFX-r2-Ec-bioC-3_at 11.33189964
AFFX-r2-Ec-bioD-5_at 14.01099968
AFFX-r2-Ec-bioD-3_at 15.07419968
AFFX-r2-P1-cre-5_at 15.62959957
AFFX-r2-P1-cre-3_at 16.08609962
AFFX-r2-Bs-lys-3_at 2.978019953
AFFX-r2-Bs-thr-5_s_at 2.169450045

Total number of rows: 25995

Table truncated, full table size 594 Kbytes.




Supplementary file Size Download File type/resource
GSM300681.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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