|
| Status |
Public on Dec 31, 2008 |
| Title |
Mutant_liver_4 |
| Sample type |
RNA |
| |
|
| Source name |
Liver
|
| Organism |
Mus musculus |
| Characteristics |
Strain: mixed
Tissue: liver
Age: 28 days
|
| Growth protocol |
Standard housing, feeding, and water. Fasted for 5 hours prior to euthanasia.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
mirVana™ miRNA Isolation Kit (Ambion)
|
| Label |
Biotin
|
| Label protocol |
100ng of total RNA per sample was amplified and biotinylated using the MessageAmp Premier kit (Ambion)
|
| |
|
| Hybridization protocol |
The hybridization was performed according to the Affymatrix manual using a Tecan HS4800 hybridization station (Tecan, Austria)
|
| Scan protocol |
Slides were scanned by a ScanArray 4000 XL scanner (Packard Biochip Technologies, USA)
|
| Description |
Hepatic gene expression data
|
| Data processing |
Image analysis was carried out using the ImaGene 6.1.0 software (BioDiscovery, Inc., USA)
|
| |
|
| Submission date |
Jun 26, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Joshua Friedman |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Pennsylvania School of Medicine
|
| Department |
Pediatrics
|
| Street address |
ARC 1007B, 3615 Civic Center Blvd.
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE11899 |
Gene expression in Dicer-deficient mouse liver |
|
| Relations |
| Reanalyzed by |
GSE119085 |
