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Sample GSM3020011 Query DataSets for GSM3020011
Status Public on Jul 31, 2018
Title RNA_Seq_28C_CT54
Sample type SRA
 
Source name Drosophila S2 cells
Organism Drosophila melanogaster
Characteristics cell type: S2 cells
time: CT54
temperature: 28C
Treatment protocol Cells were entrained by temperature cycles and were transferred to constant temperature (28 degrees Celsius). RNA was extracted at the indicated time points after the end of the entrainment.
Growth protocol Drosophila S2 cells were grown Schneider medium using standard cell culture methods.
Extracted molecule total RNA
Extraction protocol Standard RNA extraction with Zymo Direct-zolâ„¢ RNA kits
Illumina TrueSeq platform compatible dual-indexed multiplex library protocol was followed.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 4000
 
Description Sample 31
rRNA-depleted RNA
S2_genes_28C.fpkm_table.txt
S2_genes_28C.attr_table.txt
Data processing Tag mapping with TopHat2
Subsampling of aligned reads with Samtools to reach a maximum of 1 million read per sample (Sample 21 to Sample 40)
Quantification with Cufflinks software suite
Genome_build: dm3
Supplementary_files_format_and_content: Cufflinks output files
 
Submission date Feb 22, 2018
Last update date Jul 31, 2018
Contact name Guillaume Rey
E-mail(s) [email protected]
Organization name University of Geneva
Street address Campus Biotech, Chemin des Mines 9
City Geneva
ZIP/Postal code 1202
Country Switzerland
 
Platform ID GPL21306
Series (1)
GSE102495 Metabolic oscillations on the circadian time scale in Drosophila cells lacking clock genes
Relations
BioSample SAMN08580955
SRA SRX3734125

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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