|
| Status |
Public on Jul 31, 2018 |
| Title |
RNA_Seq_28C_CT66 |
| Sample type |
SRA |
| |
|
| Source name |
Drosophila S2 cells
|
| Organism |
Drosophila melanogaster |
| Characteristics |
cell type: S2 cells
time: CT66
temperature: 28C
|
| Treatment protocol |
Cells were entrained by temperature cycles and were transferred to constant temperature (28 degrees Celsius). RNA was extracted at the indicated time points after the end of the entrainment.
|
| Growth protocol |
Drosophila S2 cells were grown Schneider medium using standard cell culture methods.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Standard RNA extraction with Zymo Direct-zol™ RNA kits
Illumina TrueSeq platform compatible dual-indexed multiplex library protocol was followed.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Description |
Sample 35
rRNA-depleted RNA
S2_genes_28C.fpkm_table.txt
S2_genes_28C.attr_table.txt
|
| Data processing |
Tag mapping with TopHat2
Subsampling of aligned reads with Samtools to reach a maximum of 1 million read per sample (Sample 21 to Sample 40)
Quantification with Cufflinks software suite
Genome_build: dm3
Supplementary_files_format_and_content: Cufflinks output files
|
| |
|
| Submission date |
Feb 22, 2018 |
| Last update date |
Jul 31, 2018 |
| Contact name |
Guillaume Rey |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Geneva
|
| Street address |
Campus Biotech, Chemin des Mines 9
|
| City |
Geneva |
| ZIP/Postal code |
1202 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL21306 |
| Series (1) |
| GSE102495 |
Metabolic oscillations on the circadian time scale in Drosophila cells lacking clock genes |
|
| Relations |
| BioSample |
SAMN08580951 |
| SRA |
SRX3734129 |
