|
| Status |
Public on Feb 01, 2021 |
| Title |
01_MigA9+/-DB818 6H |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Murine Hoxa9-transformed MigA9 cell line treated with 2.5 µM of DB818
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Hoxa9-transformed MigA9 cell line
treatment time: 6h
|
| Treatment protocol |
Murine Hoxa9-transformed MigA9 cell line were treated or not with 2.5 µM of DB818 for 6, 12 or 24H
|
| Growth protocol |
RPMI-1640, 10% FCS, 1% of GM-CSF-conditioned media, 1% Pen/Strep, 37°C, 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
manufacturer's instructions
|
| |
|
| Channel 2 |
| Source name |
Hoxa9-transformed MigA9 cell line, untreated
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Hoxa9-transformed MigA9 cell line
exposure time: not exposed
|
| Treatment protocol |
Murine Hoxa9-transformed MigA9 cell line were treated or not with 2.5 µM of DB818 for 6, 12 or 24H
|
| Growth protocol |
RPMI-1640, 10% FCS, 1% of GM-CSF-conditioned media, 1% Pen/Strep, 37°C, 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
manufacturer's instructions
|
| |
|
| |
| Hybridization protocol |
Two-colors Mouse Whole Genome Agilent 44K 60-mer oligonucleotide Microarrays were scanned on an Agilent G2505B scanner.
|
| Scan protocol |
Images were quantified using Agilent Feature Extraction Software (version V.9.1.3.1).
|
| Data processing |
Agilent Feature Extraction Software (v 9.1.3.1) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Feb 26, 2018 |
| Last update date |
Feb 02, 2021 |
| Contact name |
frédéric leprêtre |
| E-mail(s) |
[email protected]
|
| Organization name |
Lille University
|
| Department |
CHU of Lille, CBP
|
| Lab |
plateforme de génomique fonctionnelle et structurale
|
| Street address |
Boulevard du Pr Leclercq
|
| City |
lille |
| ZIP/Postal code |
59037 |
| Country |
France |
| |
|
| Platform ID |
GPL2872 |
| Series (1) |
| GSE111109 |
Trancriptome profiling of murine Hoxa9-transformed MigA9 cell line treated or not with DB818 |
|
