|
| Status |
Public on Mar 06, 2018 |
| Title |
arr-11-056 Blood |
| Sample type |
genomic |
| |
|
| Source name |
Blood
|
| Organism |
Homo sapiens |
| Characteristics |
Sex: Male
status: Affected
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Extraction of genomic DNA from fresh whole blood was performed using a standard salt extraction.
|
| Label |
Biotin-streptavidin-green, DNP-anti-DNP-red
|
| Label protocol |
200ng of genomic DNA was whole-genome amplified in an overnight reaction at 37°C using multi-sample amplification mix 1 and 2 (MA1 and MA2) and multi-sample amplification master mix (WG-MSM). After incubation the amplified DNA was fragmented with fragmentation solution (WG-FMS), precipitated with isopropanol and precipitation solution (PM1) and resuspended in hybridization buffer (RA1).
|
| |
|
| Hybridization protocol |
RA1 resuspended DNA was loaded onto BeadChip arrays. After overnight incubation at 48°C, single-base extension and allele-specific staining was performed on a Te-Flow Flow-Through Chamber rack system (Tecan, Maennedorf, Switzerland).
|
| Scan protocol |
After allele-specific staining BeadChip arrays were coated with XC4/ethanol , dried for 1 hour and scanned on a BeadArray Reader and iScan System (Illumina).
|
| Description |
Genomic DNA extracted from whole blood was genotyped using Infinium Omni1-Quad Genotyping BeadChips (Illumina).
|
| Data processing |
Genomic DNA extracted from whole blood was genotyped using Infinium HumanOmni1-Quad BeadChips (Illumina).
|
| |
|
| Submission date |
Feb 26, 2018 |
| Last update date |
Mar 07, 2018 |
| Contact name |
Barbara Vona |
| E-mail(s) |
[email protected]
|
| Phone |
+49 931-31-84244
|
| Organization name |
University of Wuerzburg
|
| Department |
Institute of Human Genetics
|
| Lab |
Haaf Lab
|
| Street address |
Biozentrum, Am Hubland
|
| City |
Wuerzburg |
| State/province |
Bavaria |
| ZIP/Postal code |
97074 |
| Country |
Germany |
| |
|
| Platform ID |
GPL24663 |
| Series (1) |
| GSE111131 |
Hereditary Hearing Loss SNP-Microarray Pilot Study |
|
