|
| Status |
Public on Feb 02, 2009 |
| Title |
emb6D_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
embryo
|
| Organism |
Oryza sativa |
| Characteristics |
embryo
|
| Growth protocol |
Rice (Oryza sativa, japonica cultivar Zhonghua 11), was cultivated at day/night temperatures (28/22 °C) in phytotron
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the TRIzol method (Invitrogen).
|
| Label |
Biotin
|
| Label protocol |
RNA was labeled for use with the Affymetrix GeneChip Rice Genome array according to the manufactures protocol. (Expression Analysis Technical Manual, 2004, Affymetrix).
|
| |
|
| Hybridization protocol |
Affymetrix® GeneChip® Operating Software Version 1.0 was used for washing, staining, and scanning as described in the supplier’s protocol.
|
| Scan protocol |
Scanning procedures of the arrays were carried out using Affymetrix GeneChip Scanner 3000 as described in the Affymetrix technical manual.
|
| Description |
embryo of seed collected at 6 days after anthesis (DAA)
|
| Data processing |
The data was analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Jul 02, 2008 |
| Last update date |
Feb 01, 2009 |
| Contact name |
Hong-Wei Xue |
| E-mail(s) |
[email protected]
|
| Organization name |
Institute of Plant Physiology and Ecology,Shanghai Institutes of Biological Sciences
|
| Street address |
300 Fenglin Road
|
| City |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
| |
|
| Platform ID |
GPL2025 |
| Series (1) |
| GSE11966 |
Expression data from rice embryo,endosperm,root,leaf and seedling |
|
