|
| Status |
Public on Mar 05, 2018 |
| Title |
hPSCs undifferentiated at day0, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
hPSC, non treated, day0
|
| Organism |
Homo sapiens |
| Characteristics |
Stage: day 0
strain: hPSC hiPSC11a
marker: n/a
treatment: Serum-free- mTeSR
|
| Treatment protocol |
hPS were plated at approximatively at 30,000 cells/cm2 in mTeSR, and after recovery medium was changed to a DMEM-based medium supplemented with insulin-transferrin- selenium (ITS, Gibco), 3 μMCHIRON99021 (Axon MedChem, Tocris) (C medium) and additionally 0.5 μM LDN-193189 (Axon MedChem, Stemgent) (CL medium). On day 3, 20 ng/ml FGF2 (R&D Systems) was added .
|
| Growth protocol |
Undifferentiated hPSCs were cultured on a Matrigel (Corning)/mTESR1 system (StemCell Technologies)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from PSC populations using Trizol (Invitrogen) or with the RNAeasy plus mini-kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA targets were prepared from total RNA using a double amplification protocol according to the GeneChip® Expression Analysis Technical Manual: Two-Cycle Target Labeling Assay (P/N 701021 Rev.5, Affymetrix, Santa Clara, USA).
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| |
|
| Hybridization protocol |
Following fragmentation, cRNAs were hybridized for 16 hours at 45oC on GeneChip® Human Genome arrays. Each microarray was then washed and stained on a GeneChip® fluidics station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000 7G. Raw data (.CEL Intensity files) were extracted from the scanned images using the Affymetrix GeneChip® Command Console (AGCC) version 3.1.
|
| Description |
Gene expression data from undifferentiated hPSC
|
| Data processing |
The data were analyzed with GC-RMA
|
| |
|
| Submission date |
Mar 05, 2018 |
| Last update date |
Mar 05, 2018 |
| Contact name |
Jerome Chal |
| Organization name |
Brigham and Women's Hospital
|
| Street address |
77 ave Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL16686 |
| Series (2) |
| GSE39615 |
Transcriptomic landscape of developing Presomitic Mesoderm (PSM) from Tailbud to somite in E9.5 mouse embryo and in in vitro differentiated Paraxial mesoderm derived from mouse embryonic stem cells (mESCs). |
| GSE111391 |
Expression data of hPSCs differentiated into Paraxial mesoderm |
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