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Sample GSM30465

Query DataSets for GSM30465

Status

Public on Sep 15, 2004

Title

G-PBMC monocyte 2

Sample type

RNA

Source name

CD14+ cells from G-CSF mobilized peripheral blood

Organism

Homo sapiens

Extracted molecule

total RNA

Description

CD14+ cells were isolated from peripheral blood mononuclear cells collected from normal donors after 3 days of G-CSF stimulation. Dako Tuek4 antibody and Rat anti-mouse IgG2a+b magnetic bead selection were used to isolate cells (>95% purity). Total RNA was isolated with Qiagen RNeasy columns and converted to biotin-labeled cRNA by standard Affymetrix protocols. Targets were produced using standard Affymetrix procedures from about 12ug of total RNA. For analysis with MAS5 the sample was scaled to an arbitrary value of 1000 by overall hybridization intensity to allow comparison of multiple samples.
Keywords = monocyte, CD14, GPBMC

Submission date

Sep 10, 2004

Last update date

May 27, 2005

Contact name

Lynn Graf

E-mail(s)

[email protected]

Phone

206-667-4545

Fax

206-667-5978

URL

http://none

Organization name

Fred Hutchinson Cancer Research Center

Department

Clinical Research Division

Lab

Torok-Storb

Street address

1100 Fairview Ave. N. D1-100

City

Seattle

State/province

ZIP/Postal code

98109

Country

USA

Platform ID

GPL80

Series (1)

GSE1746

CD14+ cell analysis

Data table header descriptions
ID_REF
VALUE	'signal', a measure of the abundance of a transcript
ABS_CALL	the call in an absolute analysis that indicates if the transcript was present (P), absent (A) or marginal (M)
DETECTION P-VALUE	'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF	VALUE	ABS_CALL	DETECTION P-VALUE
A28102_at	196	A	0.139482
AB000114_at	155.5	P	0.026111
AB000115_at	745.9	P	0.002556
AB000220_at	297.2	P	0.00034
AB000381_s_at	9.2	A	0.897835
AB000409_at	2014	P	0.000225
AB000410_s_at	97.9	A	0.783476
AB000449_at	236.4	A	0.102165
AB000450_at	468.2	P	0.006871
AB000460_at	2308.5	P	0.00039
AB000462_at	298.6	A	0.123572
AB000464_at	532.9	P	0.033677
AB000466_at	158.2	A	0.916408
AB000467_at	306.8	A	0.670996
AB000468_at	1553.9	P	0.000509
AB000584_at	1039.8	A	0.275146
AB000816_s_at	13.4	A	0.794268
AB000895_at	253.7	A	0.645547
AB000896_at	340.2	A	0.368438
AB000897_at	46.3	A	0.686277

Total number of rows: 7129

Table truncated, full table size 193 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM30465.cel.gz	2.3 Mb	(ftp)(http)	CEL