|
| Status |
Public on Nov 25, 2008 |
| Title |
Liver 24hr vehicle 1 |
| Sample type |
RNA |
| |
|
| Source name |
liver
|
| Organism |
Mus musculus |
| Characteristics |
Male B6/CBF1J
|
| Treatment protocol |
liver samples were dissected then flash frozen with liquid nitrogen
|
| Extracted molecule |
total RNA |
| Extraction protocol |
frozen liver samples (~100 mg) were homogenized with a Polytron homogenizer in 4mls RLT buffer (RNAeasy/Qiagen) with 10 ul/ml BME; 600 ul processed with Qiagen Rneasy mini columns
|
| Label |
biotin
|
| Label protocol |
5 ug total RNA was used as a template for ds cDNA synthesis using Life /Technologies Superscript II cDNA synthesis kit. All of the ds cDNA was used for the labeling reaction using the ENZO Bioarray HighYield RNA Transcript Labeling Kit (T7) (42655)
|
| |
|
| Hybridization protocol |
10 ug of biotinylated cRNA were hybridized to MG_U74Av2 GeneChips for 16 hours at 45oC. Washing and staining was done using GeneChip Fluidics Station 450 EukGE-WS2v3.
|
| Scan protocol |
The GeneChips were scanned with the Agilent GeneArray Scanner
|
| Description |
vechicle treatment
|
| Data processing |
MAS5 scaled to 300. To calculate fold change values, intensity values less then 50 were adjusted to 50.
|
| |
|
| Submission date |
Jul 17, 2008 |
| Last update date |
Nov 25, 2008 |
| Contact name |
James E Fischer |
| E-mail(s) |
[email protected]
|
| Phone |
(860) 715-0704
|
| Organization name |
Pfizer, Inc
|
| Department |
Exploratory Medicinal Sciences
|
| Lab |
Dullea Lab
|
| Street address |
M/S 8118D-3036
|
| City |
Groton |
| State/province |
CT |
| ZIP/Postal code |
06340 |
| Country |
USA |
| |
|
| Platform ID |
GPL81 |
| Series (1) |
| GSE12147 |
Molecular characterization of novel peroxisome proliferator-activated receptor alpha agonists |
|
