|
| Status |
Public on Nov 16, 2018 |
| Title |
day3-PC-cell-RNA.rep3 |
| Sample type |
SRA |
| |
|
| Source name |
antigen stimulated day3 PCs
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6
cell type: primary lymphocytes
stimulation: antigen stimulated day3 PCs
genotype/variation: wild type
tissue: spleen
developmental stage: 8 weeks
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total RNA from at least 50,000 isolated mouse B cells was extracted by Trizol (Life Technology) and precipitated by RNase-free glycogen(Life technology). Immediately, we isolated mRNA from total RNA with NEBNext® Poly(A) mRNA Magnetic isolation Module(E7490S).
high throughput sequencing library was constructed with NEBNext® Ultra™ II RNA Library Prep Kit for Illumina®(E7770S).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Basecalls performed by Annoroad Co. Ltd. (naïve B and Day2 B) / BerryGenomics Co. Ltd. (Day3 B and day3 PCs)
RNA-Seq data were mapped by STAR aligner v2.5.2b and quantitified by Cufflinks and Cuffdiff.
Genome_build: GENCODE M12 (mm10)
Supplementary_files_format_and_content: The transcription levels (RPKM) were quantified with Cufflinks (v2.0.2) software.
|
| |
|
| Submission date |
Mar 26, 2018 |
| Last update date |
Nov 16, 2018 |
| Contact name |
Zhuqiang Zhang |
| E-mail(s) |
[email protected]
|
| Organization name |
Institute of Biophysics, CAS
|
| Street address |
15 Datun Road, Chaoyang District.
|
| City |
Beijing |
| State/province |
--- |
| ZIP/Postal code |
100101 |
| Country |
China |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE112351 |
Dominant roles of B cells in the initiation of CD4 T cell response to a virus-derived nanoparticle antigen |
|
| Relations |
| BioSample |
SAMN08797460 |
| SRA |
SRX3849285 |
