|
| Status |
Public on Apr 23, 2019 |
| Title |
HCT116_sh-PNO1_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Colorectal cancer cells, transduced with lentivirus encoding sh-PNO1
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HCT116
shRNA: PNO1 shRNA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol (Thermo Fisher Scientific) extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA (Cat. AM1792, Ambion).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip PrimeView Human gene expression array with constant rotation at the speed of 60 rpm. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip scanner 3000
|
| Description |
Gene expression data from HCT116 cells tranduced with lentivirus encoding PNO1 sh-RNA
|
| Data processing |
The data were analyzed with RMA using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Apr 23, 2018 |
| Last update date |
Apr 23, 2019 |
| Contact name |
Jun Peng |
| E-mail(s) |
[email protected]
|
| Organization name |
Fujian University of Traditional Chinese Medicine
|
| Department |
Academy of Integrative Medicine
|
| Street address |
1 Qiuyang Road, Minhou Shangjie
|
| City |
Fuzhou |
| State/province |
Fujian |
| ZIP/Postal code |
350122 |
| Country |
China |
| |
|
| Platform ID |
GPL15207 |
| Series (1) |
| GSE113514 |
Expression data from HCT116 cell transduced with lentivirus encoding PNO1 shRNA (sh-PNO1) or Control shRNA (sh-Ctrl) |
|
