|
| Status |
Public on Aug 30, 2018 |
| Title |
Tbet+iTregPDL1 gene replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
T cells
|
| Organism |
Mus musculus |
| Characteristics |
tissue: spleen
|
| Treatment protocol |
None
|
| Growth protocol |
Sorted CD4+Tbet+FoxP3- cells expanded in the presence of anti-CD3, antiCD28, rmIL2 and rhTGF-b1 for 3 days in the presence or absence of PDL1 followed by expansion with IL-2 and TGF-b1 for additional 4 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extracted with RNAeasy kit from Qiagen and reverse transcribed with Ambion WT expression kit
|
| Label |
biotin
|
| Label protocol |
Affymetrix (WT) terminal labelling kit
|
| |
|
| Hybridization protocol |
Affymetrix (WT) Array hybridization protocol
|
| Scan protocol |
Data were collected using Affymetrix AGCC software.
|
| Description |
Sorted CD4+Tbet+FoxP3- cells expanded in the presence of anti-CD3, antiCD28, rmIL2 and rhTGF-b1 and PDL-1 fc for 3 days followed by expansion with IL-2 and TGF-b1 for additional 4 days.
|
| Data processing |
The Partek Genomics Suite software using RMA algorithm was used.The normalized expression values are log2-scaled.
|
| |
|
| Submission date |
Apr 27, 2018 |
| Last update date |
Aug 30, 2018 |
| Contact name |
Eric W Dahlstrom |
| E-mail(s) |
[email protected]
|
| Phone |
406-375-9770
|
| Organization name |
NIAID, NIH
|
| Department |
RTB
|
| Lab |
RTS
|
| Street address |
903 S. 4th St
|
| City |
Hamilton |
| State/province |
MT |
| ZIP/Postal code |
59840 |
| Country |
USA |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE113815 |
PD-1 through asparaginyl endopeptidase regulates FoxP3 Stability in Induced Regulatory T cells |
|
| Relations |
| Alternative to |
GSM3120515 (exon level analysis) |
