|
| Status |
Public on Aug 21, 2008 |
| Title |
7360 microaerobiosis PSY 5 |
| Sample type |
RNA |
| |
|
| Source name |
B. japonicum fixJ mutant grown microaerobically
|
| Organism |
Bradyrhizobium japonicum |
| Characteristics |
Genotype: 7360
Growth condition: microaerobiosis
Medium: PSY
|
| Growth protocol |
Cells were grown to mid-exponential phase which corresponded to an OD600 of 0.4 to 0.5 in microoxic cultures (PSY-arabinose medium) and a OD600 of 0.175 to 0.225 in anoxic cultures (YEM-nitrate medium); soybean nodules were harvested 21 days post inoculation (dpi)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted as described by Hauser et al. (2007) and Pessi et al. (2007)
|
| Label |
biotin
|
| Label protocol |
2.2 ug (culture-grown bacteria) and 5.5-8 ug (nodules) cDNA were labeled with biotin
|
| |
|
| Hybridization protocol |
Modification of Affymetrix protocol used for Pseudomonas aeruginosa
|
| Scan protocol |
Standard Affymetrix procedures
|
| Description |
B. japonicum fixJ mutant grown microaerobically
|
| Data processing |
GeneChip Operating Software (GCOS) version 1.2 (Affymetrix)
|
| |
|
| Submission date |
Aug 19, 2008 |
| Last update date |
Aug 21, 2008 |
| Contact name |
Stefan Zoller |
| E-mail(s) |
[email protected]
|
| Organization name |
ETH Zurich
|
| Department |
Genetic Diversity Centre
|
| Street address |
Universitatstrasse 16
|
| City |
Zurich |
| ZIP/Postal code |
8092 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL3401 |
| Series (2) |
| GSE12491 |
Comprehensive assessment of the regulons controlled by the FixLJ-FixK2-FixK1 cascade in Bradyrhizobium japonicum |
| GSE21400 |
Autoregulation of fixK2 gene expression in Bradyrhizobium japonicum |
|
