|
| Status |
Public on May 16, 2018 |
| Title |
ctrl_GFP_neg_rep1 [1C30N] |
| Sample type |
SRA |
| |
|
| Source name |
RNA-seq of CD8T cells sorted GFP - from control
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6
genotype/variation: control
tissue: Spleen
cell type: CD8T cells
cell subtype: GFP negative
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA from sorted cells was isolated using RNA Plus Micro kit(Qiagen)
Libraries were prepared using the SMARTer Ultra low RNA kit(Clontech Laboratories)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 1000 |
| |
|
| Description |
1C30N
|
| Data processing |
Sequenced libraries were mapped to mm0 reference genome using RNA-seq unified mapper(RUM)
Read counts were quantified using RefSeq transcripts
Downstream processing and differential analysis was done using Deseq2
Genome_build: mm9
Supplementary_files_format_and_content: RNA counts file before normalization
|
| |
|
| Submission date |
May 15, 2018 |
| Last update date |
May 16, 2018 |
| Contact name |
E JOHN WHERRY |
| Organization name |
University of Pennsylvania
|
| Department |
Systems Pharmacology and Translational Therapeutics,Institute for Immunology
|
| Street address |
421 Curie Blvd,354 BRB II/III
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL15103 |
| Series (1) |
| GSE114482 |
Transcriptional profiles of miR-155 overexpressing CD8T cells during chronic infection |
|
| Relations |
| BioSample |
SAMN09210846 |
| SRA |
SRX4084646 |
