|
| Status |
Public on Jan 01, 2016 |
| Title |
Osteosarcoma cell line - CL (expression) |
| Sample type |
RNA |
| |
|
| Source name |
Osteosarcoma cell line
|
| Organism |
Homo sapiens |
| Characteristics |
Sample type: cell line
|
| Growth protocol |
Commercially available osteosarcoma cell lines were grown according to the supplier’s recommendations.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol and chloroform. Purification was performed on RNAeasy columns according to manufacturer’s instructions
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA was prepared using Ambion TotalPrep RNA Amplification Kit starting with 200 ng total RNA
|
| |
|
| Hybridization protocol |
Hybridization, washing and scanning were performed according to the manufacturer's directions
|
| Scan protocol |
Hybridization, washing and scanning were performed according to the manufacturer's directions
|
| Description |
Tumor sampls were obtained from the tissues bank at the Laboratory of Oncology Research of the Rizzoli Orthopaedic Institute, Bologna, Italy
|
| Data processing |
Expression data were normalized by the VST-RSN method using the lumi R package available at Bioconductor
|
| |
|
| Submission date |
Aug 21, 2008 |
| Last update date |
Jan 01, 2016 |
| Contact name |
Yuelin Jack Zhu |
| E-mail(s) |
[email protected]
|
| Phone |
(240)760-7439
|
| Organization name |
NCI
|
| Department |
Genetics Branch, Center for Cancer Research
|
| Lab |
Dr. Paul Meltzer's Lab
|
| Street address |
9000 Rockville Pike
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL7192 |
| Series (2) |
| GSE12512 |
Expression Profiling in Human Osteosarcoma |
| GSE12805 |
Osteosarcoma (expression, HD aCGH, aCGH) |
|
